6/19/2023 0 Comments Day 26 a new day production![]() We show that deletion of HNF1B, a transcription factor linked to congenital kidney defects, interferes with tubulogenesis, validating our experimental system for studying renal developmental biology. A comparison with fetal human kidneys suggests that day-14 organoid tissue most closely resembles late capillary loop stage nephrons. Our organoids develop tubular structures by day 8 and show optimal tissue morphology at day 14. To overcome some of these issues, we have established a simple, inexpensive, and robust method to grow kidney organoids in bulk from human induced pluripotent stem cells. Current protocols are technically complex, suffer from poor reproducibility, and have high reagent costs that restrict scalability. ![]() ![]() Kidney organoids made from pluripotent stem cells have the potential to revolutionize how kidney development, disease, and injury are studied.
0 Comments
Leave a Reply. |